🔬 Enhancing Forensic Investigations with Next Generation Sequencing (NGS)
🚀 NGS Technology Offers Several Advantages
- 🧬 Increased power of discrimination
➤ More data across the 20 CODIS Core Loci. - 🧪 Improved performance
➤ Effective with degraded DNA, low quantity DNA, or complex mixtures. - 🧭 Phenotype, ancestry, genealogy, and parentage inference
- ⚙️ High-throughput efficiency
🧫 Applications of NGS in Forensics
🧬 Nuclear DNA Analysis
- Provides size- and sequence-based info for identity markers.
- ✅ Suitable for both high-quality and degraded samples.
🔍 Case Types: Criminal investigations
🧬 Mitochondrial DNA (mtDNA) Analysis
- Used when nuclear DNA is absent or degraded.
- Provides maternal lineage info.
🔍 Case Types: Missing persons, unidentified remains, mass disasters
👁️ Externally Visible Characteristics Prediction
- Predicts physical traits using SNP markers.
🔍 Case Types: Missing persons, unidentified remains, mass disasters
👨👩👧 Kinship Testing
- Interprets relatedness using SNP markers.
🔍 Case Types: Cold cases, mass disasters, paternity testing, forensic genealogy
🧰 ForenSeq DNA Signature Prep Kit Includes:
- 🌐 Global Autosomal STRs
- 👨 Y-STRs
- 👩 X-STRs
- 🧬 Identity SNPs
- 🌍 Biogeographical Ancestry SNPs
- 👁️ Phenotypic SNPs
🕵️♂️ Investigation Workflow
- 🔍 Search Database
- 🎯 Hit / No Hit
- 👤 Suspect Included / Excluded
- 📄 Report
- 🧭 Investigative Leads
🧪 MiSeq FGx™ Forensic Genomics System
Ampath Laboratories is the first ISO 17025 accredited DNA testing lab in South Africa offering NGS-based forensic testing using the Illumina MiSeq FGx™ System.
📦 Uses the ForenSeq DNA Signature Prep Kit for a streamlined forensic workflow.
📧 Contact: forensicgenetics@ampath.co.za