Lab Update 30 – New Comprehensive Myeloid NGS Panel: Oncomine™ Myeloid Assay GX

March 2022
Ampath | ampath.co.za

Overview

Myeloid neoplasms are increasingly diagnosed, classified, and risk stratified based on their genomic profile.

Key challenges:

• Rapid evolution in precision oncology
• Need for comprehensive, timeous, and cost-effective cancer genetic testing

Ampath’s solution:

• The Oncomine™ Myeloid Assay GX (OMAGEN)
• A targeted next-generation sequencing (NGS) panel
• Detects all clinically relevant mutations and fusions in myeloid malignancies

What the Assay Covers

The OMAGEN panel includes:

✅ 53 DNA-based gene targets

• ABL1
• ANKRD26
• ASXL1
• BAALC
• BCOR
• BRAF
• CALR
• CBL
• CEBPA
• CNMT3A
• CSF3R
• DDX41
• EIF2B1
• EZH2
• FBXW2
• FLT3 (ITD/TKD)
• GATA2
• HRAS
• IDH1
• IDH2
• IKZF1
• JAK2
• KIT
• KMT2A
• KRAS
• MECOM
• MPL
• MYC
• MYD88
• NF1
• NPM1
• NRAS
• NUP98
• PHF6
• PPM1D
• PRPF8
• PSMB2
• PTPN11
• RB1
• RUNX1
• SETBP1
• SF3B1
• SH2B3
• SMC1A
• SMC3
• SRSF2
• STAG2
• TET2
• TP53
• TRIM27
• U2AF1
• WT1
• ZRSR

✅ 30 RNA fusion targets

• ABL1
• ALK
• BCL2
• BRAF
• CCND1
• CREBBP
• EGFR
• ETV6
• FGFR1
• FGFR2
• FUS
• HMGA2
• JAK2
• MECOM
• MET
• MILLT10
• MILLT3
• MYBL1
• MYH11
• NPM1
• NTRK3
• NUP214
• NUP98
• PDGFRA
• PDGFRB
• RARA
• RBM15
• RUNX1
• TCF3
• TFE3

The panel includes all mutations and rearrangements from the 2016 WHO classification of AML with recurrent genetic abnormalities, reducing the need for additional FISH testing.

Clinical Indications

Use OMAGEN in the diagnosis, classification, and monitoring of:

• Acute Myeloid Leukaemia (AML)
• Chronic Myeloid Leukaemia (CML)
• Myeloproliferative Neoplasms (MPNs)
• Clonal Eosinophilia
• Myelodysplastic Syndromes (MDS)
• Other myeloid malignancies

Test Information

• Test mnemonics:
  • OMAGEN – for routine test requests
  • OMAHOLD – for DNA/RNA extraction on hold
• Specimen type:
  • One EDTA tube containing either:
    • Bone marrow aspirate, or
    • Peripheral blood
• Sample handling:
  • Samples must arrive at the lab within 72 hours of collection for RNA extraction
• Turnaround time:
  • 10 working days (2 weeks)
• Cost (2022 cash price):
  • R13,519.00

📧 For more information or to arrange testing, contact ngs@ampath.co.za